81.   Biopsy specimens were processed immediately.

82.   Biopsy specimens were washed in saline, and homogenised in an ice cold buffer using a Teflon glass homogeniser.

83.   Biopsy specimens from control subjects and patients with ulcerative colitis were studied.

84.   All biopsy specimens were cultured on Columbia based blood agar under microaerophilic conditions for four days.

85.   Three antral biopsy specimens were taken -- one each for culture, histology, and CLO test.

86.   Biopsy specimens were fixed in formalin and examined using haematoxylin and eosin, Giemsa, and Warthin-Starry staining techniques.

87.   The patients were given supplementation for one month and colonic biopsy specimens were taken before and at the end of the trial.

88.   Colonic biopsy specimens were taken before and at the end of the supplementation period.

89.   The absence of a low folate value should not deter the physician from obtaining a duodenal biopsy specimen.

90.   The opportunity should be used to obtain duodenal biopsy specimens.