51. Additional ileal biopsy specimens from eight patients and eight peripheral lymph nodes removed for diagnostic purposes were included. 52. The uptake of PT-gliadin was monitored by immunofluorescence microscopy of intestinal biopsy specimens, using affinity purified PT-gliadin antibodies. 53. A biopsy specimen was taken under radiographic control from the jejunum of a healthy individual at the ligament of Treitz. 54. Before the instillation of PT-gliadin, a biopsy specimen was taken and studied under a steromicroscope in order to indicate a normal jejunal villous structure. 55. Biopsy specimens were submitted to histopathology examination and to phospholipid extraction. 56. In these conditions, the platelet activating factor acetylhydrolase activity which is naturally present in biopsy specimens is inactivated. 57. Ethanolic extraction is now a common method to extract platelet activating factor from various organs including biopsy specimens. 58. Two observers independently assessed the EGF staining of the biopsy specimens without information about the histological status. 59. The percentage of positively stained papillae containing EGF endothelium in each biopsy specimen was calculated as a percentage of the total number of papillae. 60. Several antral and fundic biopsy specimens were taken during the gastroscopies. |