71.   Buccal epithelial cells from eight healthy laboratory staff were prepared using established methods.

72.   This adhesion was not inhibited by D-mannose and was demonstable with both HeLa cells and human buccal epithelial cells.

73.   In contrast, we were unable to show consistent adhesion of control strains to any preparation of buccal epithelial cells from a panel of eight donors.

74.   It has been suggested that increased permeability is the result of changes in the epithelial cell mediated immune response.

75.   In contrast, only epithelial cells from ulcerative colitis showed an appreciable increase after calcium ionophore induction.

76.   Cytospin preparations of purified epithelial cells were stained by immunoperoxidase to ascertain purity and contaminating mononuclear cells.

77.   There was no evidence of platelet contamination in the lamina propria mononuclear cell or epithelial cell preparations.

78.   PAF activity was present in extracts of both epithelial cells and lamina propria mononuclear cells, spontaneously, as well as after induction by CaI.

79.   After stimulation, however, a different pattern of PAF inducation was seen between epithelial cells and lamina propria mononuclear cells.

80.   Control epithelial cells failed to respond to CaI, while cells from both forms of inflammatory bowel disease augmented their PAF activity.