31. Millimolar concentrations of stable oxidants cause a dose dependent increase in mucosal permeability in animal models and are toxic to intestinal epithelial cells. 32. Also, apoptotic degeneration was frequent within epithelial cells of remaining crypts further increasing the similiarity to graft v host disease. 33. Gastric metaplasia was defined as the occurrence of foci of gastric epithelial cells containing apical PAS positive neutral mucin together with the absence of a brush border. 34. This preparation has been shown not to affect epithelial cell proliferation. 35. Gall bladder epithelial cells serve numerous biological functions and abnormalities in their function are important in the pathogenesis of several gall bladder diseases. 36. This study reports a reliable and reproducible method of harvesting and culturing gall bladder epithelial cells. 37. This method proved reliable and reproducible for the culture of gall bladder epithelial cells and should allow direct studies of the biological properties of these cells in human tissue. 38. Furthermore, study of the immunologial characteristics of gall bladder and biliary epithelial cells should help elucidate the pathogenesis of biliary tree disorders. 39. Until recently, all studies on gall bladder epithelial cells have been restricted to using part or whole of the gall bladder. 40. More recently, other research workers used human and animal gall bladders to study aspects of epithelial cell function in relation to gall stone formation. |