1.   This increase in water solubility made possible an invitro incubation test with intraperitoneal pus or fluid from patients with peritonitis.

2.   Intraperitoneal pus or fluid was obtained for bacterial culture examination and PABA-UDCA disulphate incubation test soon after laparotomy.

3.   This increase in water solubility made possible an in vitro incubation test with intraperitoneal pus or fluid from patients with peritonitis.

4.   Positive results in this incubation test indicate the intraperitoneal presence of cholyglycine hydrolase, namely intraperitoneal leakage of intestinal bacteria.