1. Products of the chain reaction were fractionated by size in agarose gels to detect the presence of insertions or deletions. 2. This has no effect on detection of the PCR product on agarose gels, but renders the product useless for further amplification. 3. This is then electrophoresed on an agarose gel and transferred to nitrocellulose. 4. DNA from transgenic mice was digested with restriction enzymes and electrophoresed through agarose gels to determine the integrity of the construct as well as copy number. 5. PCR products were electrophoresed on agarose gels and purified by the Geneclean procedure followed by direct sequencing. 6. The PCR products were electrophoresed on agarose gels. 7. DNA samples were electrophoresed on agarose gels and transferred to nylon membranes. |
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