1.   If the mutation creates or destroys a restriction site then this can be simply examined in the products of the reaction.

2.   Several deletion mutants were prepared by exploiting singular restriction sites in the cDNA to enable progressive sequencing.

3.   Incomplete restriction sites produced on the junctions with linkers and adapters are indicated by enzymes with asterisks.

4.   Only relevant restriction sites are indicated in the expanded regions.

5.   Restriction sites and exons are shown.

6.   The same result was obtained with a transformed procyclic line in which integration was achieved using a unique MluI restriction site in the tubulin intergenic region.

7.   The restriction sites EcoRI and NcoI were used to generate the ble gene specific fragment for the run-on transciption assays.