1.   The hexane step was repeated, the decanted hexane pooled, dried on a rotary evaporator and stored.

2.   The extracted supernatants were pooled, in a clean round bottom flask and dried using a rotary evaporator.

3.   The methanolic eluates were pooled in the original round bottomed flask and dried on a rotary evaporator.

4.   The unconjugated fractions were dried on a rotary evaporator and taken up in methanol as required.