31.   RNA from several different cell lines was probed by Northern blot for the RNAs indicated to the left of the figure.

32.   DNA was isolated from cell lines or tissues by established methods.

33.   Nuclei were isolated from cell lines or spleen from transgenic animals or inbred mouse strains.

34.   Since there are large populations of non-neuronal cells in brain tissue, we used a neuronal cell line to determine the position of these sites.

35.   Nuclei were prepared from an NF-L expressing and non-expressing human cell lines and subjected to limited DNaseI digestion.

36.   To be able to analyse the promoter deletion constructs in neuronal cells, we tested a number of cell lines for their transfection efficiency.

37.   And because the cell lines are in private hands, intellectual property issues must be resolved before government-financed scientists can work with them.

38.   And it could be months, at a minimum, before their experiments determine the usefulness of existing cell lines.

39.   And she said the government wanted to detail the characteristics of each cell line, some of them fully developed and others in earlier stages.

40.   And the fact that only a few companies in the world own the cell lines also prompted fears of price gouging and preferential treatment.