11. Fourth, under our assay conditions in the presence of DNA, the dominant c-Jun kinase in unfractionated crude nuclear extracts is the DNA-PK. 12. Jun-Core is phosphorylated by a DNA-dependent kinase in HeLa nuclear extracts. 13. The high molecular weight proteins phosphorylated in this experiment are proteins endogenous to the nuclear extract. 14. Nuclear extracts were prepared from rat brain or HeLa cells as previously described. |