1.   In patients negative to challenge there was no increase in the number of specific antibody secreting cells against beta lactoglobulin and casein.

2.   By contrast, in patients negative to challenge the mean number of immunoglobulin secreting cells decreased during the challenge period.

3.   A distinct rise in the number of specific antibody secreting cells to beta lactoglobulin and casein was detected in patients reacting positively to challenge in the IgM class.

4.   In the present study a shpar rise in immunoglobulin secreting cells was measured during a clinically positive milk challenge, reflecting non-antigen specific immune response.