1.   To further test the specificity of the polypeptides detected in the crude extract we utilised the LZ competition assay.

2.   The two c-Jun derivatives were then tested for an ability to be phosphorylated by kinases present in a crude HeLa cell nuclear extract.

3.   Taken together, these data indicate that a DNA-dependent kinase present in crude HeLa nuclear extracts specifically phosphorylates the bZIP region of c-Jun.

4.   This protein was then purified and tested for an ability to be phosphorylated in a crude HeLa cell nuclear extract.

5.   Fourth, under our assay conditions in the presence of DNA, the dominant c-Jun kinase in unfractionated crude nuclear extracts is the DNA-PK.

6.   She warned against anyone trying to use a crude extract from creosote for self-treatment, because of possible toxicity.